Title:Haemoglobin Reading [α48Leu>Pro; HBA2: c.146T>C]: a comparison of different HbA1c methods in its detection

Author:Beverley Pullon

Abstract:We report a second occurrence of haemoglobin (Hb) Reading [a48Leu>Pro; HBA2: c.146T>C], and like the initial case, this was associated with normal haematological parameters. Mass spectrometry showed the variant a-chain was expressed at 21% of the total A- chain material and this was consistent with normal isopropanol stability. The Hb variant was picked up as an interfering component on HbA1c testing using cation-exchange high performance liquid chromatography (HPLC). Variable results returned from an HbA1c quality control survey highlights the difficulty of detecting Hb Reading, demonstrating how this haemoglobin variant may be under reported.
Key words: Haemoglobin Reading, haemoglobin variant, HPLC, capillary electrophoresis, HbA1c.
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