Title:Association of the methionine synthase gene polymorphism with recurrent miscarriage in Mazandaran province, Iran

Author:Zahra Asadnejad, Majid Alipour and Seyedeh Elham Norollahi

Abstract:Purpose: Methionine synthase is one of the main regulatory enzymes for homocysteine metabolism. The A2756G polymorphism in the methionine synthase gene has been implicated in recurrent miscarriage risk. The present case- control study was conducted to determine whether there is an association between the methionine synthase
A2756G polymorphism and recurrent miscarriage in Mazandaran province, north of Iran.
Methods: In this case-control study, 60 women with a previous history of at least three recurrent miscarriages and 100 healthy women without a history of miscarriage were analysed for methionine synthase A2756G polymorphism using PCR-RFLP method. The results obtained by estimating the genotype of each polymorphism were analysed using Excel software SPSS v16. Chi-square test was used to analyse the data.
Results: Twenty-four (40%) and thirty-four women (34%) in the case and control groups respectively were heterozygous for methionine synthase A2756G polymorphism. Additionally, GG (homozygous mutant) genotypes in the case and control groups were five (8%) and two (2%) respectively. Thus, frequencies of mutant GG and AG genotypes for methionine synthase polymorphisms was significantly different between the controls and cases. G allele frequency (28%) in the patient group was higher than the control group (19%). The case group, however, had significantly higher prevalence of the AG and GG genotypes (p=0.002) than the control group. Conclusion: Our study suggests that methionine synthase A2756G polymorphisms are associated with the risk of recurrent miscarriage in the northern Iranian population.
Keywords: methionine synthase, hyperhomocysteinemia, miscarriage, polymorphism, methionine synthase.
«Back         Download this article as PDF