Title:Comparison of the NG Biotech NG-Test Carba 5 and CORIS BioConcept RESIST-4 O.K.N.V. immunochromatographic lateral flow assays for the detection of carbapenemase enzymes in Enterobacterales

Author:Bronwyn Davison

Abstract:Objective: To evaluate and compare the NG-Test CARBA 5 and RESIST-4 O.K.N.V. immunochromatographic lateral flow assays for the detection of carbapenemase enzymes in Enterobacterales. Both assays have the ability to detect four of the most widespread carbapenemases; OXA-48-like, NDM, KPC, and VIM. In addition, the NG-Test CARBA 5 is able to detect IMP types. The importance of rapid diagnostic tests in microbiology laboratories is becoming increasingly crucial with the emergence of Carbapenemase-producing Enterobacterales (CPE) in New Zealand as these isolates have limited treatment options.
Methods: NG-Test CARBA 5 and RESIST-4 O.K.N.V. were performed with 58 Enterobacterales isolates with reduced susceptibility to meropenem, including 45 CPE and 13 non-CPE.
Results: The respective sensitivity results for the NG-Test CARBA 5 and RESIST-4 O.K.N.V. were as follows: OXA- 48-like - 94.7 (18/19) and 89.5% (17/19), NDM - 95.2% (20/21) and 90.5% (19/21), KPC - 100% (4/4) and 75.0%
(3/4), VIM - 100% (2/2) and 100% (2/2). Additionally, the NG-Test CARBA 5 detected 80.0% (4/5) of IMP-types. Both assays produced 100% specificity .
Conclusions: NG-Test CARBA 5 and RESIST-4 O.K.N.V. detect the main carbapenemase types in Enterobacterales, as currently found in New Zealand. They are both highly specific assays and produced overall sensitivity values of 94.1% and 89.1% respectively. These products are robust, user-friendly kits that provide results within 20 minutes (including setup and incubation) indicating the presence or absence of singular or multiple carbapenemase type(s) detected.
Keywords: carbapenemase, Enterobacterales, immunochromatographic lateral flow assay, carbapenem.
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